1. 2mL of hydrogen peroxide and 8mL of water were added into a 10mL graduated cylinder to create a hydrogen peroxide solution of 20% concentration.
2. Step 1 was repeated to make solutions of 30%, 40%, 50%, and 60% concentrations of hydrogen peroxide.
3. Overflow pan was filled with tap water.
4. A 1L graduated cylinder was filled with water and inverted into the pan. The volume of air inside the graduated cylinder was recorded.
5. 5 disks of filter paper that were immersed in beef liver were placed at the bottom of a 20mL Erlenmeyer flask. The flask was capped with a rubber stopper that has a funnel and tubing.
6. The end of the tube was put into the overflowing pan and inserted into the 1L graduated cylinder. The 20% hydrogen peroxide solution was pured into the funnel. The funnel opening was immediately blocked and the flask was shaken to allow mixing of the hydrogen peroxide and the liver.
7. New volume of gas in the 1L graduated cylinder was recorded. The initial volume was subtracted from this recording to calculate the amount of oxygen gas displaced.
8. Steps 4-7were repeated for 30%, 40%, 50%, and 60% solutions of hydrogen peroxide.
Data Table:
Sources of Errors:
- inconsistent amounts of reactants and enzymes used
- O2 gas may have escaped
- leftover water in the flask due to rinsing after each trial may influence result
